Bacterial Pathogenesis, Part A Identification and Regulation of Virulence Factors
Virginia L. Clark, Patrik M. Bavoil
This volume of Methods in Enzymology presents methods for the isolation and identification of bacterial pathogens and associated virulence determinants. It will enable the researcher to determine if closed genes or isolated gene products are involved in virulence. Major Topics Covered Include* Animal model system to determine bacterial virulence* Epidemiological techniques for the identification of bacterial strains and species* Protocols for the purification of subcellular bacterial products frequently associated with virulence* Determination of the means by which bacteria acquire iron* Methods to generate mutants, to construct isogenic strains by allelic exchange, to identify bacterial genes only expressed during infection of the host, and to study regulation of selected bacterial virulence factors* Methods for the assay of destructive bacterial enzymes and toxins
年:
1994
出版:
1
出版社:
Academic Press
语言:
english
页:
826
ISBN 10:
0121821366
系列:
Methods In Enzymology 235
文件:
PDF, 59.04 MB
IPFS:
,
english, 1994